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(ceng364)[2007](s)fianl~PPSpider^_10032.pdf
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DEPARTMENT OF CHEMICAL ENGINEERING
Hong Kong University of Science and Technology
Final Examination - Spring Semester 2007



CENG 364 C BIOMOLECULAR ENGINEERING
Thursday May 31st 2007
Time: 4.30-7.30 2464 (Lift 25/26)



Instructions to Students

(a)
Write your NAME, STUDENT ID, COURSE CODE and QUESTION NUMBER on each answer sheet.

Answer ALL questions

(b)
Answer Question 1 on the examination paper


(c)
Write EACH ANSWER for Questions 2-7 only on the RIGHT-HAND PAGES of the examination booklet


(d)
There are a total of 7 Questions . The total marks for the examination is 160 marks. The marks for each question and the recommended maximum time allocation for each question are stated for each question.


(e)
DO NOT use books or notes other than those provided during the examination.


(f)
Return the exam paper with your answers.


(g)
Read questions carefully


(h)
Some biochemical information and data is provided in the Appendix


(i)
Total number of pages including this cover page is thirteen (13)

















Each part of Question 1 is worth 3 Marks.
TOTAL MARKS = 30
Recommended MAXIMUM Time Allocation: 34 minutes

Question 1:

(a) Outline the major steps involved in undertaking a Metabolic Flux Analysis (M.F.A.) (3 Marks) .

(b) Describe three methods available for reducing the production of undesired inhibitory catabolic end-products (3 Marks).

(c) Why is YATP (g biomass / mol ATP) considered the most suitable comparison for the efficiency of biomass production. Why is it a better basis than yXS (growth yield C g biomass / g substrate) (3 Marks)?

(d) List three major limitations of Michaelis-Menten kinetics (3 Marks).

(e) What unique features of cellular reactions catalysed by enzymes make the calculation of G difficult. Give three examples of modifications to the normal chemical thermodynamics definition of G when considering these types of reactions (3 Marks).

(f) Describe the major features of the generation of ATP form NAD(H) using the electron transport chain (3 Marks).

(g) What are the major assumptions related to the metabolic engineering use of the flux control coefficients (3 Marks).

(h) What is the difference between steady-state and equilibrium for a metabolic pathway consisting of:
k1 kp
A B C

k2
(3 Marks)









(i) Name three major roles for the Pentose Phosphate Pathway (3 Marks):

1.
_____________________________________________________




2.
_____________________________________________________




3.
_____________________________________________________




(j) Which of the following two pathways would lead to the lower cell from substrate yield? (3 Marks)

(Please circle one